ASV phylogenetic tree

Context

This is a bioinformatics tool used to reconstruct phylogenetic trees from ASV (Amplicon Sequence Variant) sequences. It allows researchers to infer evolutionary relationships among microbial sequences based on their similarity. Phylogenetic trees are essential for diversity analyses, community comparisons, and ecological interpretation in microbial studies.

How it does

The program uses a FASTA file of ASV seed sequences and an abundance BIOM file as input. It constructs a phylogenetic tree, taking into account sequence similarities to infer branching relationships. It is designed for datasets with fewer than 10,000 sequences to ensure computational efficiency. Logs and HTML reports are generated to summarize the tree-building process and provide graphical visualization of results.

Configuration: 16S V3V4 Swarm


mkdir FROGS/SWARM/
sbatch -J tree -o LOGS/tree.out -e LOGS/tree.err -c 8 --export=ALL --wrap="module load devel/Miniforge/Miniforge3 && module load bioinfo/FROGS/FROGS-v5.0.2 && tree.py --input-fasta FROGS/SWARM/filters.fasta --input-biom FROGS/SWARM/affiliation.biom --output-tree FROGS/SWARM/phylo_tree.nwk --log-file FROGS/SWARM/tree.log --html FROGS/SWARM/tree_results.html && module unload bioinfo/FROGS/FROGS-v5.0.2"
(to see all settings: tree.py --help)



Interpretation: 16S V3V4 Swarm

This report shows that all ASVs are in the tree.
You can use the tree view output to explore the tree.
fastqc_per_sequence_quality_scores_plot