FROGS Func


Welcome to the FROGS Func tutorials!
FROGS Func extends your analyses beyond ASV generation and taxonomic affiliation, enabling the functional inference of microbial communities.
Using the PICRUSt2 suite, you’ll learn how to:

  • Place ASVs within a phylogenetic tree and estimate gene copy numbers.
  • Predict functional abundances at the gene family level.
  • Infer metabolic pathways, allowing higher-level interpretation of community functions.

These modules complement the FROGS Core pipeline by connecting taxonomy to function, helping you explore the metabolic potential of your communities.
Step by step, you’ll learn to use these tools on Galaxy or via command line, and to integrate functional results with other analyses to enrich your bioinformatics studies.