Welcome to the FROGS Stat tutorials!
FROGS Stat provides a complete set of tools to explore and interpret your microbial community data. With these modules, you can go beyond simple ASV tables to perform advanced ecological and statistical analyses that reveal the structure and dynamics of your communities.
With FROGS Stat, you’ll learn how to:

• Import your data into Phyloseq for integrated statistical analysis.
• Explore taxonomic composition across multiple levels.
• Assess within-sample diversity using alpha diversity metrics.
• Compare communities across samples through beta diversity analyses.
• Identify sample groups with clustering approaches.
• Investigate community structure using ordination methods.
• Test hypotheses with multivariate analysis of variance.
• Apply DESeq2 preprocessing for ASV- and function-level data.
• Generate DESeq2 visualizations to highlight differential abundance.

Together, these modules form a comprehensive framework that connects taxonomic, functional, and statistical insights, helping you gain a deeper understanding of your microbial datasets.