FROGS — Fast, Reliable, Open-source amplicon analysis.

From raw reads to taxonomic and functional insights — with built-in statistics — in just a few clicks. Accessible to everyone, experts and non-experts alike, on Galaxy or via command line.

FROGS around the world

-
Countries with data
-
Maximum number of citations in one country
-
Average citations by country

Highlights

Accessible to all

FROGS is designed for everyone — from students to expert researchers. Use it via Galaxy or command line, with step-by-step tutorials and clear documentation available in the How it works? section.

Learn More

Flexible and innovative clustering

Choose between Swarm (local thresholds) or DADA2 for precise ASV grouping — delivering more accurate results than traditional global-threshold methods.

Learn More

Supports diverse data types

FROGS handles multiplexed or demultiplexed sequences, short or long reads, and any amplicon type, offering full flexibility for a wide range of datasets.

Learn More

Proven and trusted performance

Extensively tested on 16S, ITS, and other amplicon datasets, FROGS is trusted by a broad research community for its robustness and reliability in microbiome analyses.

Learn More

Continuously updated and well supported

Actively maintained by a responsive development team, FROGS evolves with user feedback to ensure an effective, up-to-date analysis platform.

Learn More

Extended with companion tools

Enhance your analyses with a suite of companion and extension tools designed to expand FROGS’ capabilities. (See the “Extension tools” section for details.)

Learn More